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Repeat modules and N-linked glycans define structure and antigenicity of a critical enterotoxigenic E. coli adhesin .

Zachary T BerndsenMarjahan AkhtarMahima ThapaTim J VickersAaron J SchmitzJonathan L TorreSabyasachi BabooPardeep KumarNazia KhatoonAlaullah SheikhMelissa F HamrickJolene K DiedrichSalvador Martinez de BartolomePatrick T GarrettJohn Yates IiiJackson S TurnerRenee M LairdFrédéric M PolyChad K PorterJeffrey CoppsAli H EllebedyAndrew B WardJames M Fleckenstein
Published in: bioRxiv : the preprint server for biology (2024)
(ETEC), a leading cause of diarrhea disproportionately affecting young children in low-income regions, are a priority for vaccine development. Individuals possessing A blood-type are more susceptible to severe cholera-like disease. EtpA, a secreted, immunogenic, blood group A binding protein, is a current vaccine target antigen. Here, we determined the atomic structure of EtpA in complex with protective as well as non-protective monoclonal antibodies targeting two different domains of the protein, allowing us to pinpoint key regions involved in blood-group A antigen recognition and uncover the mechanism of antibody-based protection. In addition, we show through mass-spectrometry that EtpA is extensively and heterogeneously glycosylated at surface-exposed asparagine residues by a promiscuous and low-fidelity glycosyltransferase, EtpC, and that this unique form of bacterial glycosylation is critical for to development of protective immune responses. Lastly, polyclonal antibodies from vaccinated mice as well as monoclonal antibodies obtained from ETEC-infected human volunteers revealed that the highly antigenic surface of EtpA exhibits both protective and non-protective epitopes. These results greatly expand our understanding of ETEC pathogenesis, and the immune responses elicited by these common infections, providing valuable information to aid in the rational design and testing of subunit vaccines.
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