Label-Free Visualization of Carbapenemase Activity in Living Bacteria.
Ye ZhangJin-E LeiYuan HeJianhua YangWenjing WangAbdul WaseyJiru XuYue LinHaiming FanGuangyin JingCe ZhangYi JinPublished in: Angewandte Chemie (International ed. in English) (2018)
Evaluating enzyme activity intracellularly on natural substrates is a significant experimental challenge in biomedical research. We report a label-free method for real-time monitoring of the catalytic behavior of class A, B, and D carbapenemases in live bacteria based on measurement of heat changes. By this means, novel biphasic kinetics for class D OXA-48 with imipenem as substrate is revealed, providing a new approach to detect OXA-48-like producers. This in-cell calorimetry approach offers major advantages in the rapid screening (10 min) of carbapenemase-producing Enterobacteriaceae from 142 clinical bacterial isolates, with superior sensitivity (97 %) and excellent specificity (100 %) compared to conventional methods. As a general, label-free method for the study of living cells, this protocol has potential for application to a wider range and variety of cellular components and physiological processes.
Keyphrases