Login / Signup

Single-Sided Competitive Axial Coordination of G-Quadruplex/Hemin as Molecular Switch for Imaging Intracellular Nitric Oxide.

Lei ZhangJun ZhouFengjiao MaQuanbo WangHui XuHuangxian JuJianping Lei
Published in: Chemistry (Weinheim an der Bergstrasse, Germany) (2018)
Axial coordination is a crucial biological process to regulate biomolecules' functions in natural enzymes. However, it is a great challenge to determine the single or dual axial interaction between the metal center of enzymes and the ligand. In this work, a controllable axial coordination system was developed based on G-quadruplex/hemin complex by designing a series of fluorescent derivatives. The mechanism on axial coordination of G-quadruplex/hemin with coumarin-imidazole ligands was proposed to be single-sided, and led to fluorescence quenching of ligands. Upon addition of nitric oxide, the fluorescence of ligands was recovered through competitive axial coordination, providing a "signal on" strategy for signal transduction. More significantly, the fluorescent imaging of intracellular nitric oxide was achieved after conjugating with gold nanoparticles. Also, the proposed protocol provided a smart strategy to monitor the relationship between nitric oxide and p53 protein activity in living cells.
Keyphrases
  • nitric oxide
  • living cells
  • single molecule
  • fluorescent probe
  • gold nanoparticles
  • nitric oxide synthase
  • hydrogen peroxide
  • high resolution
  • randomized controlled trial
  • amino acid