Simultaneous analysis of derivatized allyl isothiocyanate together with its phase II metabolites by ultra-high-performance liquid chromatography/tandem mass spectrometry.
Hiu-Lok NganTao WangYi RuYanyan ChenCharlie LiZongwei CaiPublished in: Rapid communications in mass spectrometry : RCM (2022)
An analytical method involving a rapid derivatization pre-treatment and quantitative UHPLC/ESI-QqQ-MS/MS analysis has been developed for a biological sample assay of AITC, enabling selective and sensitive detection of the AITC derivative and AITC metabolites simultaneously. The method developed could be applied to the DMPK study of AITC as well.
Keyphrases
- ms ms
- liquid chromatography tandem mass spectrometry
- sensitive detection
- phase ii
- clinical trial
- loop mediated isothermal amplification
- high resolution
- open label
- quantum dots
- simultaneous determination
- high throughput
- ultra high performance liquid chromatography
- combination therapy
- liquid chromatography
- double blind
- water soluble
- gas chromatography mass spectrometry
- single cell