Application of a RiboTag-based approach to generate and analyze mRNA from enteric neural cells.

Patrick Leven Reiner SchneiderKevin D SiemensWalker Scot Jackson Sven Wehner

Published in: Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society (2021)

We present a robust and selective protocol that allows the generation of cell type-specific transcriptional in vivo snapshots of distinct enteric cell populations that will be especially useful for various intestinal disease models involving peripheral neural cells.

Keyphrases

induced apoptosis
cell cycle arrest
randomized controlled trial
single cell
transcription factor
stem cells
cell death
oxidative stress
signaling pathway
binding protein
mesenchymal stem cells
bone marrow
genetic diversity
heat stress