Tibolone Preserves Mitochondrial Functionality and Cell Morphology in Astrocytic Cells Treated with Palmitic Acid.
Yeimy González-GiraldoLuis Miguel Garcia-SeguraValentina EcheverriaGeorge E BarretoPublished in: Molecular neurobiology (2017)
Obesity has been associated with increased chronic neuroinflammation and augmented risk of neurodegeneration. This is worsened during the normal aging process when the levels of endogenous gonadal hormones are reduced. In this study, we have assessed the protective actions of tibolone, a synthetic steroid with estrogenic actions, on T98G human astrocytic cells exposed to palmitic acid, a saturated fatty acid used to mimic obesity in vitro. Tibolone improved cell survival, and preserved mitochondrial membrane potential in palmitic acid-treated astrocytic cells. Although we did not find significant actions of tibolone on free radical production, it modulated astrocytic morphology after treatment with palmitic acid. These data suggest that tibolone protects astrocytic cells by preserving both mitochondrial functionality and morphological complexity.
Keyphrases
- induced apoptosis
- cell cycle arrest
- oxidative stress
- type diabetes
- metabolic syndrome
- endoplasmic reticulum stress
- fatty acid
- insulin resistance
- weight loss
- cell death
- single cell
- traumatic brain injury
- body mass index
- physical activity
- big data
- cell therapy
- deep learning
- induced pluripotent stem cells
- virtual reality