Construction of a new thermophilic fungus Myceliophthora thermophila platform for enzyme production using a versatile 2A peptide strategy combined with efficient CRISPR-Cas9 system.
Fangya LiQian LiuXiaolin LiChenyang ZhangJingen LiWenliang SunDandan LiuDongguang XiaoChaoguang TianPublished in: Biotechnology letters (2020)
The 2A peptide approach worked well in M. thermophila and can be used to heterologously co-express two different proteins, and thus in combination with efficient CRISPR-Cas system will accelerate establishing hyper-secretion platforms for biotechnological applications.