Keratin 17 and Collagen type 1 genes: Esophageal cancer molecular marker discovery and evaluation.
Hui-Wen PanJie HongAizhong ShaoZhiguo ZhaoGuowen DingZhijie FangKeping ChenJingfeng ZhuPublished in: The clinical respiratory journal (2024)
One hundred eighty pairs of tissues of esophageal squamous cell carcinoma (ESCC) were tested by the transcriptome sequencing in order to explore etiology factors. The chi-square test and correlation analysis demonstrated that the relative expression levels of keratin 17 (KRT17) and collagen type I α1 chain (COL1A1) were significantly higher in EC with diabetes. Expression of KRT17 was correlated with blood glucose (r = 0.204, p = 0.001) and tumor size (r = -0.177, p = 0.038) in patients. COL1A1 correlated with age (r = -0.170, p = 0.029) and blood glucose levels (r = 0.190, p = 0.015). Experimental results of qRT-PCR: KRT17 and COL1A1 genes were highly expressed in ESCC (p < 0.05). When the two genes were used as a combination test, the positive detection rate of EC was 90.6%, and the ROC curve had greater power. The KRT17 and COL1A1 genes had the potential to be biomarkers for the diagnosis of ESCC.
Keyphrases
- blood glucose
- genome wide
- glycemic control
- poor prognosis
- bioinformatics analysis
- genome wide identification
- type diabetes
- end stage renal disease
- ejection fraction
- gene expression
- blood pressure
- cardiovascular disease
- chronic kidney disease
- newly diagnosed
- single cell
- small molecule
- prognostic factors
- high throughput
- transcription factor
- adipose tissue
- skeletal muscle
- real time pcr
- label free
- quantum dots