Involvement of 2-deoxyglucose-6-phosphate phosphatases in facilitating resilience against ionic and osmotic stress in Saccharomyces cerevisiae .
Chinmayee AwasthyZeinab Abdelmoghis HefnyWouter Van GenechtenUwe HimmelreichPatrick Van DijckPublished in: Microbiology spectrum (2024)
, which are induced as part of the general stress response pathway, but their natural substrate in the cells remains unclear. They are known to dephosphorylate the non-natural substrate 2-deoxyglucose-6-phosphate. Here, we show that overexpression of these genes overcomes the osmosensitive phenotype of mutants that are unable to produce glycerol. However, in these overexpression strains, very little glycerol is produced indicating that the Dog enzymes do not seem to be involved in a previously predicted alternative pathway for glycerol production. Our work shows that overexpression of the DOG genes may improve osmotic and ionic stress tolerance in yeast.
Keyphrases
- saccharomyces cerevisiae
- cell proliferation
- positron emission tomography
- transcription factor
- genome wide
- induced apoptosis
- genome wide identification
- bioinformatics analysis
- escherichia coli
- cell cycle arrest
- diabetic rats
- stress induced
- climate change
- social support
- signaling pathway
- heat stress
- gene expression
- depressive symptoms
- cell death
- pet ct
- wild type