Identification of protein lysine methylation readers with a yeast three-hybrid approach.
Agnieszka Anna Rawłuszko-WieczorekFranziska KnodelRaluca TamasArunkumar DhayalanAlbert JeltschPublished in: Epigenetics & chromatin (2018)
Our approach introduces a cost-effective method for screening reading domains binding to histone and nonhistone proteins which is not limited by expression levels of the candidate reading proteins. Identification of already known and novel H3K9me3 readers proofs the power of the Y3H assay which will allow for proteome-wide screens of PTM readers.