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Generation of self-compatible diploid potato by knockout of S-RNase.

Mingwang YeZhen PengDie TangZhongmin YangDawei LiYunmei XuChunzhi ZhangSanwen Huang
Published in: Nature plants (2018)
Re-domestication of potato into an inbred line-based diploid crop propagated by seed represents a promising alternative to traditional clonal propagation of tetraploid potato, but self-incompatibility has hindered the development of inbred lines. To address this problem, we created self-compatible diploid potatoes by knocking out the self-incompatibility gene S-RNase using the CRISPR-Cas9 system. This strategy opens new avenues for diploid potato breeding and will also be useful for studying other self-incompatible crops.
Keyphrases
  • crispr cas
  • genome editing
  • climate change
  • copy number
  • gene expression
  • transcription factor