Ochratoxin A Exposure Impairs Porcine Granulosa Cell Growth via the PI3K/AKT Signaling Pathway.
Tian-Yu ZhangXiao-Feng SunLan LiJin-Mei MaRui-Qian ZhangNa LiXue-Lian LiuPaul W DyceWei ShenPublished in: Journal of agricultural and food chemistry (2019)
The mycotoxin ochratoxin A (OTA), a naturally occurring food contaminant, has a toxic effect on the growth and development of follicles in pigs. However, little is known regarding the specific toxic effects of OTA exposure on oocytes and granulosa cells (GCs). In this study, we cultured porcine ovarian GCs and exposed them to OTA in vitro in order to explore the mechanism causing the negative effects. Initially, it was found that OTA exposure inhibited cell viability in a time and dose dependent manner. We also showed that OTA exposure increased oxidative stress, decreased proliferation ratio, and increased apoptosis ratio in GCs. We revealed an important role for the PI3K/AKT signal pathway in GC proliferation and apoptosis by RNA-seq analysis. The results not only showed that OTA treatment significantly affected the expression of genes within the PI3K/AKT pathway but also demonstrated a concrete relationship between the PI3K/AKT pathway and GC cell proliferation and apoptosis. In conclusion, the results demonstrated that OTA exposure impaired porcine GC growth via the PI3K/AKT signaling pathway.
Keyphrases
- signaling pathway
- oxidative stress
- cell cycle arrest
- induced apoptosis
- pi k akt
- rna seq
- endoplasmic reticulum stress
- cell proliferation
- cell death
- single cell
- epithelial mesenchymal transition
- poor prognosis
- dna damage
- type diabetes
- gene expression
- transcription factor
- metabolic syndrome
- mass spectrometry
- genome wide
- binding protein
- polycystic ovary syndrome
- long non coding rna
- high resolution
- endothelial cells
- skeletal muscle
- diabetic rats
- gas chromatography
- dna methylation
- insulin resistance
- liquid chromatography
- tandem mass spectrometry
- data analysis