Harnessing multiplex crRNA enables an amplification-free/CRISPR-Cas12a-based diagnostic methodology for Nosema bombycis .

Huarui ZhangHuijuan ZhaoLu CaoBin YuJunhong WeiGuoqing PanJialing BaoZeyang Zhou

Published in: Microbiology spectrum (2023)

. This study provides a new approach for the inspection and quarantine of silkworm pébrine disease in sericulture and provides a new method for the detection of other pathogens.

Keyphrases

crispr cas
real time pcr
genome editing
label free
high throughput
big data
gram negative
loop mediated isothermal amplification
machine learning
artificial intelligence
quantum dots