Magnetic-activated cell sorting is not completely effective at reducing sperm DNA fragmentation.
Mercedes González MartínezPascual Sánchez-MartínMónica Dorado-SilvaJosé Luís FernándezEstibaliz GironesStephen D JohnstonJaime GosálvezPublished in: Journal of assisted reproduction and genetics (2018)
MACS is capable of reducing the proportion of SDF, especially spermatozoa with a highly degraded DNA molecule. However, this reduction did not preclude the presence of a small subpopulation of spermatozoa with damaged DNA in the MACS- fraction. The MACS protocol was two- to threefold more efficient when the SDF in NEAT ejaculate was equal to or greater than 30%. In 4 of 20 individuals, the level of SDF after MACS resulted in semen for ICSI with a higher or non-significant reduction when compared to SDF observed in the NEAT ejaculate.