Kinetic analysis of endogenous β 2 -adrenoceptor-mediated cAMP GloSensor TM responses in HEK293 cells.

Sean A CullumDmitry B VeprintsevStephen J Hill

Published in: British journal of pharmacology (2022)

Kinetic analysis of real-time signalling data can improve our understanding of the impact of agonist-antagonist interactions at receptors expressed at low endogenous levels in different tissues. It provides valuable insights into the hemi-equilibria that can occur in low receptor reserve systems with agonist-antagonist interactions, due to incomplete dissociation of antagonist whilst the peak agonist response is developing.

Keyphrases

induced apoptosis
gene expression
binding protein
electronic health record
oxidative stress
endoplasmic reticulum stress
deep learning
cell death
data analysis
pi k akt