The adaptive immune system restrains Alzheimer's disease pathogenesis by modulating microglial function.
Samuel E MarshEdsel M AbudAnita LakatosAlborz KarimzadehStephen T YeungHayk DavtyanGianna M FoteLydia LauJason G WeingerThomas E LaneMatthew A InlayWayne W PoonMathew Blurton-JonesPublished in: Proceedings of the National Academy of Sciences of the United States of America (2016)
The innate immune system is strongly implicated in the pathogenesis of Alzheimer's disease (AD). In contrast, the role of adaptive immunity in AD remains largely unknown. However, numerous clinical trials are testing vaccination strategies for AD, suggesting that T and B cells play a pivotal role in this disease. To test the hypothesis that adaptive immunity influences AD pathogenesis, we generated an immune-deficient AD mouse model that lacks T, B, and natural killer (NK) cells. The resulting "Rag-5xfAD" mice exhibit a greater than twofold increase in β-amyloid (Aβ) pathology. Gene expression analysis of the brain implicates altered innate and adaptive immune pathways, including changes in cytokine/chemokine signaling and decreased Ig-mediated processes. Neuroinflammation is also greatly exacerbated in Rag-5xfAD mice as indicated by a shift in microglial phenotype, increased cytokine production, and reduced phagocytic capacity. In contrast, immune-intact 5xfAD mice exhibit elevated levels of nonamyloid reactive IgGs in association with microglia, and treatment of Rag-5xfAD mice or microglial cells with preimmune IgG enhances Aβ clearance. Last, we performed bone marrow transplantation studies in Rag-5xfAD mice, revealing that replacement of these missing adaptive immune populations can dramatically reduce AD pathology. Taken together, these data strongly suggest that adaptive immune cell populations play an important role in restraining AD pathology. In contrast, depletion of B cells and their appropriate activation by T cells leads to a loss of adaptive-innate immunity cross talk and accelerated disease progression.
Keyphrases
- high fat diet induced
- gene expression
- inflammatory response
- bone marrow
- clinical trial
- lipopolysaccharide induced
- immune response
- mouse model
- magnetic resonance
- neuropathic pain
- nk cells
- wild type
- type diabetes
- computed tomography
- randomized controlled trial
- magnetic resonance imaging
- cognitive decline
- signaling pathway
- open label
- artificial intelligence
- big data
- metabolic syndrome
- cognitive impairment
- study protocol
- combination therapy
- deep learning
- smoking cessation
- data analysis
- pi k akt
- genetic diversity
- endoplasmic reticulum stress