Rutin-loaded liquid crystalline nanoparticles attenuate oxidative stress in bronchial epithelial cells: a PCR validation.
Meenu MehtaKeshav Raj PaudelShakti Dhar ShuklaMadhur D ShastriSaurabh SatijaDinesh Kumar ChellapanMonica GulatiHarish DurejaFlavia C ZacconiPhilip Michael HansbroDinesh Kumar ChellappanKamal DuaPublished in: Future medicinal chemistry (2021)
Aim: In the present study, the inhibitory potential of rutin-loaded liquid crystalline nanoparticles (LCNs) on oxidative stress was determined in human bronchial epithelial cells (BEAS-2B) by analysing the expression levels of different antioxidant (NADPH quinine oxidoreductase-1 (NQO1); γ-glutamyl cysteine synthetase catalytic subunit (GCLC)) and pro-oxidant (NADPH oxidase (Nox)-4; Nox2B) genes. Results: Our findings revealed that the rutin-loaded LCNs inhibited the genes, namely Nox2B and Nox4, which caused oxidative stress. In addition, these nanoparticles demonstrated an upregulation in the expression of the antioxidant genes Gclc and Nqo-1 in a dose-dependent manner. Conclusion: The study indicates the promising potential of rutin-loaded LCNs as an effective treatment strategy in patients with high oxidant loads in various respiratory diseases.
Keyphrases
- oxidative stress
- drug delivery
- poor prognosis
- anti inflammatory
- reactive oxygen species
- cancer therapy
- dna damage
- genome wide
- ischemia reperfusion injury
- induced apoptosis
- wound healing
- diabetic rats
- endothelial cells
- ionic liquid
- room temperature
- signaling pathway
- gene expression
- genome wide identification
- single cell
- human health
- smoking cessation