Long-read sequencing for fast and robust identification of correct genome-edited alleles: PCR-based and Cas9 capture methods.
Christopher V McCabePeter D PriceGemma F CodnerAlasdair J AllanAdam CaulderSkevoulla ChristouJorik LoefflerMatthew MackenzieElke MalzerJoffrey MiannéKrystian J NowickiEdward J O'NeillFran J PikeMarie HutchisonBenoit Petit-DemoulièreMichelle E StewartHilary GatesSara E WellsNicholas D SandersonLydia TeboulPublished in: PLoS genetics (2024)
Targeted long-read sequencing supports in-depth characterisation of all experimental models that aim to produce knock-in or conditional alleles, including those that contain a mix of genome-edited alleles. PCR- or Cas9 capture-based modalities bring different advantages to the analysis.