MicroRNA-582-5p suppresses non-small cell lung cancer cells growth and invasion via downregulating NOTCH1.
Jianghong LiuShengshuo LiuXiaoyan DengJiaoyu RaoKaiyuan HuangGengrui XuXiaokang WangPublished in: PloS one (2019)
Non-small cell lung cancer (NSCLC) is the most common cancer worldwide. MicroRNAs have been shown to be correlated with biological processes of various tumors. In this study, we observed that the expression of miR-582-5p was lower in NSCLC tissues than that in para-carcinoma tissues. Ectopic expression of miR-582-5p significantly inhibited NCI-H358 cell proliferation and invasion. Knockdown of miR-582-5p showed the opposite results, with cell growth rate and the invasive capacity of PC-9 cells enhanced. Furthermore, we elucidated that NOTCH1 is a target of miR-582-5p and there is an inverse correlation between miR-582-5p and NOTCH1 expression in NSCLC tissues. Overexpression of NOTCH1 in miR-582-5p-overexpressing NCI-H358 cells could partially reverse the inhibition of cell proliferation and invasion by miR-582-5p. Thus, our research demonstrated that miR-582-5p suppresses NSCLC cell lines' growth and invasion via targeting oncoprotein NOTCH1 and restoration of miR-582-5p might be feasible therapeutic strategy for NSCLC.
Keyphrases
- small cell lung cancer
- cell proliferation
- advanced non small cell lung cancer
- poor prognosis
- induced apoptosis
- gene expression
- brain metastases
- cell cycle arrest
- signaling pathway
- single cell
- cell migration
- binding protein
- endoplasmic reticulum stress
- squamous cell carcinoma
- stem cells
- transcription factor
- cancer therapy
- pi k akt
- mesenchymal stem cells
- young adults
- tyrosine kinase