CRISPR-dCas13-tracing reveals transcriptional memory and limited mRNA export in developing zebrafish embryos.

Youkui HuangBao-Qing GaoQuan MengLiang-Zhong YangXu-Kai MaHao WuYu-Hang PanLi YangDong LiLing-Ling Chen

Published in: Genome biology (2023)

This optimized dCas13-based toolkit enables robust spatial-temporal tracking of endogenous mRNAs and uncovers features of transcription and mRNP motion, providing a powerful toolkit for endogenous RNA visualization in a multicellular developmental organism.

Keyphrases

transcription factor
crispr cas
genome wide
genome editing
gene expression
working memory
high speed
dna methylation
mass spectrometry
genome wide analysis