Login / Signup

IgG3 subclass antibodies recognize antigenically drifted influenza viruses and SARS-CoV-2 variants through efficient bivalent binding.

Marcus J BoltonClaudia P ArevaloTrevor GriesmanShuk Hang LiPaul BatesPatrick C WilsonScott E Hensley
Published in: bioRxiv : the preprint server for biology (2022)
Influenza viruses and coronaviruses undergo continuous change, successfully evading human antibodies elicited from prior infections or vaccinations. It is important to identify features that allow antibodies to bind with increased breadth. Here we examined the effect that different IgG subclasses have on monoclonal antibody binding and neutralization. We show that IgG subclass is a determinant of antibody breadth, with IgG3 affording increased neutralization of antigenically drifted variants of influenza virus and SARS-CoV-2. Future studies should evaluate IgG3 therapeutic antibodies and vaccination strategies or adjuvants that may skew antibody responses toward broadly reactive isotypes.
Keyphrases
  • sars cov
  • monoclonal antibody
  • copy number
  • endothelial cells
  • dna binding
  • transcription factor
  • pluripotent stem cells