Single-cell transcriptomics reveals functionally specialized vascular endothelium in brain.
Hyun-Woo JeongRodrigo Diéguez-HurtadoHendrik ArfJian SongHongryeol ParkKai KruseLydia M SorokinRalf H AdamsPublished in: eLife (2022)
Through single-cell RNA sequencing of non-neuronal cell populations of the murine cerebral cortex during development, adulthood, ageing, and neuroinflammation, we identify reactive endothelial venules, a compartment of specialized postcapillary endothelial cells that are characterized by consistent expression of cell adhesion molecules, preferential leukocyte transmigration, association with perivascular macrophage populations, and endothelial activation initiating CNS immune responses. Our results provide novel insights into the heterogeneity of the cerebral vasculature and a useful resource for the molecular alterations associated with neuroinflammation and ageing.
Keyphrases
- single cell
- cerebral ischemia
- endothelial cells
- subarachnoid hemorrhage
- cell adhesion
- blood brain barrier
- rna seq
- immune response
- brain injury
- palliative care
- high throughput
- high glucose
- poor prognosis
- nitric oxide
- traumatic brain injury
- depressive symptoms
- functional connectivity
- vascular endothelial growth factor
- lipopolysaccharide induced
- adipose tissue
- genetic diversity
- lps induced
- toll like receptor
- bone marrow
- peripheral blood
- cognitive impairment
- single molecule
- mesenchymal stem cells
- white matter
- cerebral blood flow