Fabrication of 6-gingerol, doxorubicin and alginate hydroxyapatite into a bio-compatible formulation: enhanced anti-proliferative effect on breast and liver cancer cells.
Danushika C ManatungaRohini M De SilvaK M Nalin de SilvaDulharie T WijeratneGathsaurie Neelika MalavigeGareth WilliamsPublished in: Chemistry Central journal (2018)
Ample attention has been devoted to the construction of anti-cancer drug delivery systems with increased stability, and controlled and targeted delivery, minimizing toxic effects. In this study we have designed a magnetically attractive hydroxyapatite (m-HAP) based alginate polymer bound nanocarrier to perform targeted, controlled and pH sensitive drug release of 6-gingerol, doxorubicin, and their combination, preferably at low pH environments (pH 5.3). They have exhibited higher encapsulation efficiency which is in the range of 97.4-98.9% for both 6-gingerol and doxorubicin molecules whereas the co-loading has accounted for a value of 81.87 ± 0.32%. Cell proliferation assays, fluorescence imaging and flow cytometric analysis, demonstrated the remarkable time and dose responsive anti-proliferative effect of drug loaded nanoparticles on MCF-7 cells and HEpG2 cells compared with their neat counter parts. Also, these systems have exhibited significantly reduced toxic effects on non-targeted, non-cancerous cells in contrast to the excellent ability to selectively kill cancerous cells. This study has suggested that this HAP based system is a versatile carrier capable of loading various drug molecules, ultimately producing a profound anti-proliferative effect.
Keyphrases
- drug delivery
- cancer therapy
- drug release
- induced apoptosis
- cell cycle arrest
- cell proliferation
- fluorescence imaging
- magnetic resonance
- photodynamic therapy
- tissue engineering
- oxidative stress
- cell death
- wound healing
- autism spectrum disorder
- emergency department
- drug induced
- intellectual disability
- single cell
- lactic acid