Antioxidative and Skin Protective Effects of Canarium subulatum Methanol Extract on Keratinocytes.
So-Hyeon HwangJi Hye KimEunju ChoiSang Hee ParkYoung-Jin SonPublished in: Evidence-based complementary and alternative medicine : eCAM (2021)
Canarium subulatum is a traditional medical herb used in South Asia. Recently, the anti-inflammatory effects of C. subulatum methanol extract (Cs-ME) have been reported; however, the effect of Cs-ME on skin physiology has not yet been elucidated. Therefore, in this study, we evaluated the protective effect of Cs-ME on UV-induced skin aging and cell death as well as the reinforcing effect on the skin barrier. According to viable cell counting and MTT assays, Cs-ME significantly reduced UV-evoked HaCaT cell death. Cs-ME blocked reactive oxygen species (ROS) generation in UV-irradiated HaCaT cells and showed radical scavenging activity against DPPH and ABTS. In addition, H2O2-induced cell death was inhibited by Cs-ME, indicating that Cs-ME protects cells from UV-derived cell death through the suppression of ROS. PCR analysis revealed that Cs-ME diminished the expression of aging-related HYAL-1 and MMP-1 genes in UV-treated HaCaT cells. Elevated HYAL-1 and MMP-1 mRNA expression in H2O2-stimulated HaCaT cells was also decreased by Cs-ME, suggesting that Cs-ME exerts antiaging activity via the inhibition of ROS. Expression of skin barrier components including filaggrin and hyaluronic acid synthase-1 was increased by Cs-ME and was modulated by ERK/p38-AP-1 signaling. Collectively, our data show that Cs-ME has cytoprotective and antiaging activity based on antioxidant properties. Furthermore, Cs-ME exerts skin barrier protective ability by regulating the AP-1 signaling pathway. Therefore, Cs-ME has the potential for use as an ingredient in cosmetics to protect the skin from UV irradiation, prevent photoaging, and strengthen the skin barrier.
Keyphrases
- cell death
- cell cycle arrest
- induced apoptosis
- anti inflammatory
- signaling pathway
- wound healing
- soft tissue
- reactive oxygen species
- healthcare
- oxidative stress
- poor prognosis
- hyaluronic acid
- stem cells
- transcription factor
- endoplasmic reticulum stress
- risk assessment
- deep learning
- single cell
- epithelial mesenchymal transition
- long non coding rna
- dna methylation
- diabetic rats
- endothelial cells
- electronic health record
- stress induced