High-content screen in human pluripotent cells identifies miRNA-regulated pathways controlling pluripotency and differentiation.

Ildercílio Mota de Souza LimaJosiane Lilian Dos Santos SchiavinatoSarah Blima Paulino LeiteDanuta SastreHudson Lenormando de Oliveira BezerraBruno SangiorgiAmanda Cristina CorveloniCarolina Hassibe ThoméVitor Marcel FaçaDimas Tadeu CovasMarco Antônio ZagoMauro GiaccaMiguel ManoRodrigo Alexandre Panepucci

Published in: Stem cell research & therapy (2019)

We present the first multiparametric high-content microRNA functional screening in human pluripotent cells. Integration of this type of data with similar data obtained from siRNA screenings (using the same HCS assay) could provide a large-scale functional approach to identify and validate microRNA-mediated regulatory mechanisms controlling pluripotency and differentiation.

Keyphrases

induced apoptosis
endothelial cells
cell cycle arrest
electronic health record
high throughput
transcription factor
induced pluripotent stem cells
endoplasmic reticulum stress
big data
pluripotent stem cells
cell death
machine learning
genome wide
gene expression
drug delivery
cell fate
data analysis