A CRISPR toolbox for generating intersectional genetic mouse models for functional, molecular, and anatomical circuit mapping.
Savannah J LuskAndrew McKinneyPatrick J HuntPaul G FaheyJay PatelAndersen ChangJenny J SunVena K MartinezPing Jun ZhuJeremy R EgbertGenevera AllenXiaolong JiangBenjamin R ArenkielAndreas S ToliasMauro Costa-MattioliRussell S RayPublished in: BMC biology (2022)
The lines presented here are representative examples of outcomes possible with the successful application of our genetic toolkit for the facile development of diverse, modifiable animal models. This toolkit will allow labs to create single or dual recombinase effector lines easily for any cell population or subpopulation of interest when paired with the appropriate Cre and FLP recombinase mouse lines or viral vectors. We have made our tools and derivative intersectional mouse and ES cell lines openly available for non-commercial use through publicly curated repositories for plasmid DNA, ES cells, and transgenic mouse lines.
Keyphrases
- genome wide
- crispr cas
- induced apoptosis
- escherichia coli
- mouse model
- single molecule
- copy number
- sars cov
- single cell
- dendritic cells
- genome editing
- regulatory t cells
- cell therapy
- type diabetes
- stem cells
- quantum dots
- oxidative stress
- dna methylation
- cell free
- gold nanoparticles
- bone marrow
- gene expression
- skeletal muscle
- mesenchymal stem cells
- gene therapy