The substrate scope of dehydratases in antibiotic biosynthesis and their application in kinetic resolutions.
Zhiyong YinDaniel BärBertolt GustJeroen S DickschatPublished in: Organic & biomolecular chemistry (2022)
Nine dehydratases involved in the biosynthesis of secondary metabolites in addition to FabZ from fatty acid biosynthesis were investigated for their substrate scope using a panel of N -acetylcysteamine (SNAC) thioesters. The best performing enzyme BorDH2 was applied in kinetic resolutions.