Increased CD4 + CD8 + Double Positive T Cells during Hantaan Virus Infection.
Huiyuan ZhangYazhen WangYing MaKang TangChunmei ZhangMeng WangXiyue ZhangManling XueXiaozhou JiaHaifeng HuNa LiZhuang RanBoquan JinLihua ChenYun ZhangYu-Si ZhangPublished in: Viruses (2022)
Hantaan virus (HTNV) infection causes an epidemic of hemorrhagic fever with renal syndrome (HFRS) mainly in Asia. It is well known that T cells mediated anti-viral immune response. Although previous studies showed that double positive T (DP T) cells, a little portion of T lymphocytes, were involved in adaptive immune response during virus infection, their kinetic changes and roles in HTNV infection have not yet been explored. In this study, we characterized DP T cells from HFRS patients based on flow cytometry data combined with scRNA-seq data. We showed that HTNV infection caused the upregulation of DP T cells in the peripheral blood, which were correlated with disease stage. The scRNA-seq data clustered DP T cells, unraveled their gene expression profile, and estimated the ordering of these cells. The production of granzyme B and CD107a from DP T cells and the abundant TCR distribution indicated the anti-viral property of DP T cells. In conclusion, this study identified, for the first time, an accumulation of DP T cells in the peripheral blood of HFRS patients and suggested these DP T cells belonging to CD8 + T cells lineage. The DP T cells shared the similar characteristics with cytotoxic T cells (CTL) and exerted an anti-viral role in HFRS.
Keyphrases
- peripheral blood
- immune response
- end stage renal disease
- newly diagnosed
- ejection fraction
- sars cov
- flow cytometry
- electronic health record
- prognostic factors
- big data
- cell proliferation
- peritoneal dialysis
- dna methylation
- signaling pathway
- poor prognosis
- induced apoptosis
- long non coding rna
- case report
- artificial intelligence
- cell death
- nk cells