Transcriptional Biomarkers for Treatment Monitoring of Pulmonary Drug-Resistant Tuberculosis: Protocol for a Prospective Observational Study in Indonesia.
Ida ParwatiDian Ayu Eka PitalokaLidya ChaidirPublished in: Tropical medicine and infectious disease (2022)
Many blood-based gene expression biomarkers for monitoring tuberculosis (TB) treatment have been suggested so far, but promising biomarker results for drug-resistant TB treatment response have not been studied. This protocol presents a prospective observational study in Indonesia to profile the human blood transcriptome for predicting the response to drug-resistant TB treatment, focusing on pulmonary TB, and to adapt the specific RNA signature to the qRT-PCR platform. Longitudinal blood samples will be collected from 44 subjects with rifampicin resistant TB, confirmed by Xpert MTB/RIF, and 52 healthy controls. RNA-Seq will be performed to identify changes in the transcriptome following TB treatment. A discriminative RNA signature will be chosen and translated into a score for use in a quantitative PCR-based assay. This study will provide crucial information to guide the discovery and design of a clinically implementable tool to monitor the response of TB treatment.
Keyphrases
- drug resistant
- mycobacterium tuberculosis
- gene expression
- multidrug resistant
- rna seq
- acinetobacter baumannii
- randomized controlled trial
- single cell
- high throughput
- emergency department
- pseudomonas aeruginosa
- dna methylation
- transcription factor
- oxidative stress
- cystic fibrosis
- combination therapy
- antiretroviral therapy
- heat shock protein