A Cas9-mediated adenosine transient reporter enables enrichment of ABE-targeted cells.

Nicholas BrookhouserToan NguyenStefan J TekelKylie Standage-BeierXiao WangDavid A Brafman

Published in: BMC biology (2020)

We established a method to detect adenosine base-editing activity within a cell, which increases the efficiency of editing at multiple genomic locations through an enrichment of edited cells. In the future, XMAS-TREE will greatly accelerate the application of ABEs in biomedical research.

Keyphrases

crispr cas
genome editing
induced apoptosis
cell cycle arrest
cell death
oxidative stress
drug delivery
mesenchymal stem cells
gene expression
bone marrow
current status
cerebral ischemia