Ras interacting protein 1 facilitated proliferation and invasion of diffuse large B-cell lymphoma cells.
Xiaojing XingXuguang WangMeichen LiuQianxue GuoHongyue WangPublished in: Cancer biology & therapy (2023)
A GTPase binding protein, Ras interacting protein 1 (RASIP1), has been reported with a tumor-promoting role in lung cancer cells, and its role in lymphoma remains unknown. The analysis of medical databank shows that RASIP1 is upregulated in diffuse large B-cell lymphoma (DLBCL) specimens. In this article, we demonstrated that RASIP1 is highly expressed in DLBCL cell lines, compared with primary B cells. The gain- and loss-of-function experiments were performed to investigate the effects of RASIP1 on DLBCL cells. CCK-8, flow cytometry, western blot, and transwell assays demonstrated that silence of RASIP1 inhibited proliferation, cell cycle transition, and invasion and induced significant apoptosis in DLBCL cells, and ectopic expression of RASIP1 played opposite roles. Xenograft results revealed that RASIP1 facilitated the growth of DLBCL cells in vivo . These findings suggest that RASIP1 may be required for malignancy of DLBCL cells. In addition, we also found that the expression of RASIP1 was negatively regulated by forkhead box O3 (FOXO3), which has been reported to suppress the proliferation of DLBCL cells. Our results indicate that FOXO3 is bound to the promoter sequence of RASIP1 and inhibits its transcription. The suppressive effects of FOXO3 on proliferation and invasion of DLBCL cells were neutralized by RASIP1. In conclusion, we demonstrate that FOXO3 negatively regulated RASIP1 facilitates growth and invasion of DLBCL cells, provides novel diagnostic markers and therapeutic targets for DLBCL in clinic.
Keyphrases
- diffuse large b cell lymphoma
- induced apoptosis
- cell cycle arrest
- transcription factor
- epstein barr virus
- signaling pathway
- cell death
- binding protein
- endoplasmic reticulum stress
- healthcare
- poor prognosis
- flow cytometry
- cell cycle
- primary care
- dna methylation
- endothelial cells
- south africa
- single cell
- cell migration
- high throughput