Analytical validation of a real-time hydrolysis probe PCR assay for quantifying Plasmodium falciparum parasites in experimentally infected human adults.
Claire Y T WangEmma L BallardZuleima PavaLouise MarquartJane GaydonSean C MurphyDavid WhileyPeter O'RourkeJames S McCarthyPublished in: Malaria journal (2021)
The 18S rRNA gene qPCR assay is specific and highly reproducible and can provide reliable and accurate parasite quantification. The assay is considered fit for use in evaluating drug efficacy in malaria clinical trials.
Keyphrases
- plasmodium falciparum
- high throughput
- clinical trial
- endothelial cells
- high resolution
- randomized controlled trial
- emergency department
- gene expression
- quantum dots
- mass spectrometry
- dna methylation
- induced pluripotent stem cells
- liquid chromatography
- drug induced
- open label
- adverse drug
- transcription factor
- genome wide identification
- real time pcr