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Metagenomic profiling of ticks: Identification of novel rickettsial genomes and detection of tick-borne canine parvovirus.

Anuradha RaviSuheir EreqatAmer Al-JawabrehZiad AbdeenOmar Abu ShammaHolly HallMark J PallenAbedelmajeed Nasereddin
Published in: PLoS neglected tropical diseases (2019)
Here, we have shown how a cost-effective two-stage protocol can be used to detect and characterise tick-borne pathogens and endosymbionts. In recovering genome sequences from an unexpected pathogen (canine parvovirus) and a previously unsequenced pathogen (Candidatus Rickettsia barbariae), we demonstrate the open-ended nature of metagenomics. We also provide evidence that ticks can carry canine parvovirus, raising the possibility that ticks might contribute to the spread of this troublesome virus.
Keyphrases
  • candida albicans
  • randomized controlled trial
  • minimally invasive
  • single cell
  • genome wide
  • loop mediated isothermal amplification
  • antibiotic resistance genes
  • dna methylation
  • microbial community
  • label free