Genome-wide CRISPR Screen Reveals RAB10 as a Synthetic Lethal Gene in Colorectal and Pancreatic Cancers Carrying SMAD4 Loss.

Hélène Erasimus Vanessa Kolnik Frédéric Lacroix Sukhvinder S Sidhu Stéphane D'Agostino Olivier Lemaitre Alexandre Rohaut Isabelle Sanchez Gilbert Thill Michel Didier Laurent Debussche Christophe Marcireau

Published in: Cancer research communications (2023)

This study identified and validated RAB10 as new synthetic lethal gene with SMAD4. This was achieved by conducting a whole-genome CRISPR screens in different colorectal and pancreatic cell lines. A future RAB10 inhibitors could correspond to a new therapeutic solution for patients with cancer with SMAD4 deletion.

Keyphrases

genome wide
transforming growth factor
epithelial mesenchymal transition
dna methylation
copy number
high throughput
gene expression
crispr cas
current status
signaling pathway
young adults
genome editing
transcription factor