The Effect of Different FSH Administration Before Ovum Pick Up on Superstimulation Response and Oocyte Yield.

This study aims to evaluate the effect of synchronization and different superstimulation protocols on oocyte yield before ovum-pick up (OPU) to provide a homogeneous follicle population. Animals in all study groups except the control group applied a synchronization protocol including modified ovsynch+progesterone and dominant follicle ablation (DFA, on the 6 th day following synchronization). In group 1, oocytes were retrieved by ultrasonography only on the 4 th day after DFA. In group 2, 250 μg pFSH (100 μg IM, 150 μg SC) was administered as a single dose on the 2 nd day following DFA, and oocytes were retrieved on the second day following this injection. In group 3, 250 μg pFSH was administered IM in four equal doses 12 hours apart on the 1 st and 2 nd days following DFA, and oocytes were retrieved 2 days after the last FSH injection. In group 4, 250 μg pFSH (IM) dissolved in Montanide ISA 206 adjuvant was administered as a single dose on the 2 nd day following DFA, and oocytes were retrieved 2 days after this treatment. In the control group (group 5), oocytes were retrieved from animals without any hormonal treatment on a random day of the estrous cycle. In order to assess the follicle population in the ovary on the day of OPU, the number of follicles according to their diameter was determined by ultrasonography in all groups. The ratio of medium-sized follicles (3-8 mm) was higher in the synchronized groups (Groups 1, 2, 3, and 4) than in the control group (Group 5) (p<0.05). It was determined that the total number of oocytes obtained after OPU and the number of oocytes of suitable quality (Grade A and B) in in vitro embryo production were higher in the synchronized groups (Groups 2, 3, and 4) compared to the control group. It was found that the number of Grade-A quality oocytes was higher in the superstimulated groups (Groups 2, 3, and 4) than the other groups. As a result, it was found that the synchronization and superstimulation treatments prior to the OPU increased the ratio of medium-sized follicles and the total number of oocytes obtained. In addition to the synchronization protocol, it was determined that superstimulation treatments increased the oocyte quality obtained with OPU. Furthermore, it was observed that a single dose of FSH dissolved in Montanide ISA 206 adjuvant produced a superstimulation response similar to that produced by repeated doses of FSH.