Ultrasound-Controlled Delivery of Growth Factor-Loaded Cerasomes Combined with Polycaprolactone Scaffolds Seeded with Bone Marrow Mesenchymal Stem Cells for Biomimetic Tendon-to-Bone Interface Engineering.
Cancan DuRuiqi WuWenqiang YanJingchao FangWenli DaiYiqun WangJin ChengXiaoqing HuYingfang AoXiaolong LiangZhenlong LiuPublished in: ACS applied materials & interfaces (2023)
Rotator cuff tear (RCT) is a prevalent shoulder injury that poses challenges for achieving continuous and functional regeneration of the tendon-to-bone interface (TBI). In this study, we controlled the delivery of growth factors (GFs) from liposomal nanohybrid cerasomes by ultrasound and implanted three-dimensional printed polycaprolactone (PCL) scaffolds modified with polydopamine loaded with bone marrow mesenchymal stem cells (BMSCs) to repair tears of the infraspinatus tendon in a lapine model. Direct suturing (control, CTL) was used as a control. The PCL/BMSC/cerasome (PBC) devices are sutured with the enthesis of the infraspinatus tendon. The cerasomes and PCL scaffolds are highly stable with excellent biocompatibility. The roles of GFs BMP2, TGFβ1, and FGF2 in tissue-specific differentiation are validated. Compared with the CTL group, the PBC group had significantly greater proteoglycan deposition ( P = 0.0218), collagen volume fraction ( P = 0.0078), and proportions of collagen I ( P = 0.0085) and collagen III ( P = 0.0048). Biotin-labeled in situ hybridization revealed a high rate of survival for transplanted BMSCs. Collagen type co-staining at the TBI is consistent with multiple collagen regeneration. Our studies demonstrate the validity of biomimetic scaffolds of TBI with BMSC-seeded PCL scaffolds and GF-loaded cerasomes to enhance the treatment outcomes for RCTs.
Keyphrases
- tissue engineering
- rotator cuff
- growth factor
- traumatic brain injury
- wound healing
- drug delivery
- stem cells
- magnetic resonance imaging
- bone mineral density
- cancer therapy
- severe traumatic brain injury
- bone regeneration
- mesenchymal stem cells
- mild traumatic brain injury
- computed tomography
- gold nanoparticles
- ultrasound guided
- pet imaging
- reduced graphene oxide
- signaling pathway
- bone marrow
- positron emission tomography