Design of Novel Mechanically Resistant and Biodegradable Multichannel Platforms for the Treatment of Peripheral Nerve Injuries.
Caterina ValentinoBarbara ViganiGaia ZuccaMarco RuggeriGiorgio MarrubiniCinzia BoselliAntonia Icaro CornagliaGiuseppina SandriSilvia RossiPublished in: Biomacromolecules (2023)
Peripheral nerve injury is one of the most debilitating pathologies that severely impair patients' life. Although many efforts have been made to advance in the treatment of such a complex disorder, successful strategies to ensure full recovery are still scarce. The aim of the present work was to develop flexible and mechanically resistant platforms intended to act as a support and guide for neural cells during the regeneration process of peripheral nerve injury. For this purpose, poly(lactic- co -glycolic acid) (PLGA)/poly(d,l-lactic acid) (PDLLA)/poly(ethylene glycol) 400 (PEG)-multichannel-based scaffolds (MCs) were prepared through a multistep process involving electrospun microfibers coated with a polymer blend solution and used as a sacrificial mold. In particular, scaffolds characterized by random (MCR) and aligned (MCA) multichannel were obtained. A design of experiments approach (DoE) was employed to identify a scaffold-optimized composition. MCs were characterized for morphological and mechanical properties, suturability, degradability, cell colonization, and in vivo safety. A new biodegradable, biocompatible, and safe microscale multichannel scaffold was developed as the result of an easy multistep procedure.
Keyphrases
- peripheral nerve
- tissue engineering
- drug delivery
- lactic acid
- end stage renal disease
- escherichia coli
- stem cells
- chronic kidney disease
- newly diagnosed
- multidrug resistant
- induced apoptosis
- combination therapy
- prognostic factors
- cell proliferation
- oxidative stress
- drug release
- cell cycle arrest
- signaling pathway
- cell death
- quality improvement
- replacement therapy
- bone regeneration