The humanized nanobody RBD-1-2G tolerates the spike N501Y mutation to neutralize SARS-CoV-2.
Ying FuJuliana da Fonseca Rezende E MelloBryan D FlemingAlex RennCatherine Z ChenXin HuMiao XuKirill GorshkovQuinlin HansonWei ZhengEmily M LeeLalith PereraRobert PetrovichManisha PradhanRichard T EastmanZina ItkinThomas StanleyAllen HsuVenkata DandeyWilliam GilletteTroy TaylorNitya RamakrishnanShelley PerkinsDominic EspositoEunkeu OhKimihiro SusumuMason WolakMarc FerrerMatthew D HallMario J BorgniaAnton SimeonovPublished in: bioRxiv : the preprint server for biology (2021)
A cost-effective, high-throughput, adaptable pipeline capable of identifying effective humanized nanobodies against SARS-CoV-2.