A novel tryptamine-appended rhodamine-based chemosensor for selective detection of Hg2+ present in aqueous medium and its biological applications.
Subhenjit HazraChandan BodhakSourav ChowdhuryDwipanjan SanyalSubhro MandalKrishnananda ChattopadhyayAnimesh PramanikPublished in: Analytical and bioanalytical chemistry (2019)
A novel rhodamine-tryptamine conjugate-based fluorescent and chromogenic chemosensor (RTS) for detection of Hg2+ present in water was reported. After gradual addition of Hg2+ in aqueous methanol solution of RTS, a strong orange fluorescence and deep-pink coloration were observed. The probe showed high selectivity towards Hg2+ compared to other competitive metal ions. The 1:1 binding stoichiometry between RTS and Hg2+ was established by Job's plot analysis and mass spectroscopy. Initial studies showed that the synthesized probe RTS possessed fair non-toxicity and effectively passed through cell walls of model cell systems, viz., human neuroblastoma (SHSY5Y) cells and cervical cells (HeLa) to detect intercellular Hg2+ ions, signifying its utility in biological system. The limit of detection (LOD) was found to be 2.1 nM or 0.42 ppb by fluorescence titration. Additionally, the potential relevance of synthesized chemosensor for detecting Hg2+ ions in environmental water samples has been demonstrated. Graphical abstract ᅟ.
Keyphrases
- aqueous solution
- fluorescent probe
- living cells
- quantum dots
- single molecule
- induced apoptosis
- cell cycle arrest
- label free
- cell therapy
- single cell
- loop mediated isothermal amplification
- oxidative stress
- ionic liquid
- depressive symptoms
- endoplasmic reticulum stress
- risk assessment
- real time pcr
- stem cells
- mass spectrometry
- human health
- transcription factor
- energy transfer
- solid state
- social support
- oxide nanoparticles
- photodynamic therapy
- bone marrow
- cell adhesion