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Definitive hematopoietic stem/progenitor cells from human embryonic stem cells through serum/feeder-free organoid-induced differentiation.

Selami DemirciJuan J Haro-MoraAlexis LeonardClaire DrysdaleDaniela MalideKeyvan KeyvanfarKhaled EssawiRaul VizcardoNaritaka TamaokiNicholas P RestifoJohn F TisdaleNaoya Uchida
Published in: Stem cell research & therapy (2020)
In summary, definitive HSPCs can be derived from ESCs through a dynamic cellular process from an organoid-like structure, where erythroid progeny are capable of producing adult hemoglobin and lymphoid progeny shows a diverse TCR repertoire.
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