Chronic Alcohol Consumption Disrupts the Skeletal Muscle Circadian Clock in Female Mice.
Abigail L TiceJoseph A LaudatoBradley S GordonJennifer L SteinerPublished in: Journal of biological rhythms (2022)
The intrinsic skeletal muscle core clock has emerged as a key feature of metabolic control and influences several aspects of muscle physiology. Acute alcohol intoxication disrupts the core molecular clock, but whether chronic consumption, like that leading to alcoholic myopathy, is also a zeitgeber for skeletal muscle remains unknown. The purpose of this work was to determine whether chronic alcohol consumption dysregulates the skeletal muscle core molecular clock and clock-controlled genes (CCGs). C57BL/6Hsd female mice (14 weeks old) were fed a control (CON) or alcohol (EtOH) containing liquid diet for 6 weeks. Gastrocnemius muscles and serum were collected from CON and EtOH mice every 4-h for 24-h. Chronic alcohol consumption disrupted genes of the core clock including suppressing the rhythmic peak of expression of Bmal1 , Per1, Per2 , and Cry2 . Genes involved in the regulation of Bmal1 also exhibited lower rhythmic peaks including Reverb α and Myod1 . The CCGs, Dbp, Lpl, Hk2 , and Hadh were also suppressed by alcohol. The nuclear expression patterns of MYOD1, DBP, and REVERBα were shifted by alcohol, while no change in BMAL1 was detected. Overall, these data indicate that alcohol disrupted the skeletal muscle core clock but whether these changes in the core clock are causative or a consequence of alcoholic myopathy requires future mechanistic confirmation.
Keyphrases
- alcohol consumption
- skeletal muscle
- insulin resistance
- high fat diet induced
- drug induced
- liver injury
- poor prognosis
- type diabetes
- late onset
- signaling pathway
- electronic health record
- gene expression
- weight loss
- physical activity
- resting state
- respiratory failure
- ionic liquid
- early onset
- intensive care unit
- endothelial cells
- hepatitis b virus
- binding protein
- functional connectivity
- preterm birth
- wild type
- acute respiratory distress syndrome