PEGylated and Non-PEGylated TCP-1 Probes for Imaging of Colorectal Cancer.
Zhonglin LiuBrian D GrayChristy BarberLi WanLars R FurenlidRongguang LiangZheng LiJames M WoolfendenKoon Y PakDiego R MartinPublished in: Molecular imaging and biology (2021)
TCP-1-based probes undergo endocytosis and localize in the cytoplasm and nucleus of human and mouse CRC cells. Tumor detectability of fluorescent TCP-1 peptide with a PEG4 spacer is promising due to its enhanced tumor binding affinity and rapid clearance kinetics from nontumor tissues. Non-PEGylated [99mTc]Tc-HYNIC-TCP-1 exhibits lower nonspecific accumulation in the liver and gastrointestinal tract and might have better capability for detecting CRC lesions in clinical sites. TCP-1 may represent an innovative targeting molecule for detecting CRC noninvasively.
Keyphrases
- living cells
- small molecule
- induced apoptosis
- endothelial cells
- single molecule
- high resolution
- gene expression
- cell cycle arrest
- drug delivery
- quantum dots
- fluorescent probe
- cancer therapy
- recombinant human
- cell death
- transcription factor
- endoplasmic reticulum stress
- dna binding
- pluripotent stem cells
- pi k akt
- label free