Phosphotungstic acid-enhanced microCT: Optimized protocols for embryonic and early postnatal mice.

Kate M LesciottoSusan M Motch PerrineMizuho KawasakiTimothy SteckoTimothy M RyanKazuhiko KawasakiJoan T Richtsmeier

Published in: Developmental dynamics : an official publication of the American Association of Anatomists (2019)

These protocols have broad applicability to mouse models for a variety of diseases and conditions. Minor experimentation in the staining duration can expand this protocol to additional age groups, permitting ontogenetic studies of internal organs and soft tissue structures within their 3D in situ position.

Keyphrases

soft tissue
mouse model
randomized controlled trial
preterm infants
high fat diet induced
high resolution
case control
flow cytometry
type diabetes
wild type
adipose tissue
insulin resistance