Intrastrain comparison of the chemical composition and antioxidant activity of an edible mushroom, Pleurotus giganteus, and its potent neuritogenic properties.
Phan Chia WeiPamela DavidYee-Shin TanMurali NaiduKah-Hui WongUmah Rani KuppusamyVikineswary SabaratnamPublished in: TheScientificWorldJournal (2014)
Two strains of Pleurotus giganteus (commercial and wild) were tested for their ability to induce neurite outgrowth in rat pheochromocytoma (PC12) and mouse neuroblastoma-2a (N2a) cells. Treatment with the mushroom extracts resulted in neuronal differentiation and neuronal elongation, but not nerve growth factor (NGF) production. Linoleic acid (4.5-5.0%, w/w) which is a major fatty acid present in the ethanol extract promoted NGF biosynthesis when augmented with low concentration of NGF (5 ng/mL). The two strains of mushroom were found to be high in protein (154-192 g kg(-1)), total polysaccharides, phenolics, and flavonoids as well as vitamins B1, B2, and B3. The total phenolics present in the mushroom extracts were positively correlated to the antioxidant activity (free radical scavenging, ferric reducing power, and lipid peroxidation inhibition). To conclude, P. giganteus could potentially be used in well-balanced diet and as a source of dietary antioxidant to promote neuronal health.
Keyphrases
- growth factor
- fatty acid
- oxidative stress
- escherichia coli
- induced apoptosis
- anti inflammatory
- cerebral ischemia
- public health
- healthcare
- mental health
- physical activity
- subarachnoid hemorrhage
- weight loss
- health information
- amino acid
- cell proliferation
- climate change
- endoplasmic reticulum stress
- binding protein
- brain injury
- blood brain barrier
- small molecule
- peripheral nerve
- cell wall