Specific recognition of Cr3+ under physiological conditions by allyl substituted appendage rhodamine and its cell-imaging studies.
Anup Kumar BhanjaSnehasis MishraKaushik NaskarSuvendu MaityKrishna Das SahaChittaranjan SinhaPublished in: Dalton transactions (Cambridge, England : 2003) (2017)
An allyloxynitrophenylimio-propylhydroxyl appended rhodamine (RD-3) specifically recognises Cr3+ in aqueous buffer medium at pH 7.4. The visible light excitable reagent (λex 505 nm) on selective binding to Cr3+ exhibited a strong fluorescence turn-on response with powerful emission at 557 nm and the tail of this luminescence band is extended to ∼650 nm. Interference studies confirm that other metal ions (27 ions) do not hamper the detection process of Cr3+. The sensing mechanism is studied by absorption, fluorescence, Job's plot, and 1H NMR titration studies. The detection limit is as low as 33 nM. The practical applicability of RD-3 is examined for imaging Cr(iii) ions in HCT116 (human colorectal carcinoma) cells.
Keyphrases
- quantum dots
- high resolution
- photodynamic therapy
- fluorescent probe
- visible light
- case control
- energy transfer
- light emitting
- endothelial cells
- single molecule
- loop mediated isothermal amplification
- single cell
- label free
- cell therapy
- real time pcr
- sensitive detection
- water soluble
- fluorescence imaging
- cell proliferation
- mass spectrometry
- pluripotent stem cells