Study of microRNAs targeted Dvl2 on the osteoblasts differentiation of rat BMSCs in hyperlipidemia environment.
Xin HuangZhifeng WangDuoduo LiZhengfei HuangXiaofei DongChuanhua LiJing LanPublished in: Journal of cellular physiology (2018)
Dishevelled 2 (Dvl-2), a key mediator of the wnt/β-catenin signaling pathway, plays critical roles in osteoblasts differentiation in hyperlipidemia environment. In our previous study, we observed a strong correlation between increased dvl2 expression and decreased new bone formation around implants in a rat hyperlipidemia implant surgery model. However, transcriptional regulation of Dvl2 by microRNAs in this process remains unknown. In the current study, we searched in online database and identified four significantly up-regulated miRNAs, miR-21-5p, miR-29c-3p, miR-138-5p, and miR-351-5p that could potentially regulate Dvl2. Using Western blot and dual-luciferase assays, we confirmed that miR29c-3p suppresses Dvl2 expression by binding to its 3'-UTR. Our results suggest a novel transcriptional regulation mechanism of Dvl2 by miR-29c-3p in osteoblasts differentiation of BMSCs.
Keyphrases
- cell proliferation
- long non coding rna
- poor prognosis
- long noncoding rna
- high fat diet
- oxidative stress
- stem cells
- type diabetes
- emergency department
- coronary artery disease
- south africa
- transcription factor
- skeletal muscle
- atrial fibrillation
- electronic health record
- metabolic syndrome
- soft tissue
- health information