Overall, the decontamination measures applied during the preparation of the amniotic tissue can be regarded as effective. We found a significant reduction of the number of microorganisms detected in the amniotic tissue following antibiotic administration.However, some of the remaining species identified in the processed samples may be considered as contamination during the preparation and testing procedures.For instance, C.acnes can be considered a result of secondary contamination due to incorrect handling. Species such as Bacillus most likely managed to endure the decontamination process owing to its natural resilience against harsh circumstances.