Proteome-wide mapping of PQS-interacting proteins in Pseudomonas aeruginosa.
Rambabu DandelaDanielle MantinBenjamin F CravattJosep RayoMichael M MeijlerPublished in: Chemical science (2018)
The opportunistic human pathogen Pseudomonas aeruginosa secretes 2-heptyl-3-hydroxy-4-quinolone (PQS), a quorum sensing (QS) signal that regulates the expression of numerous virulence genes. Here we report the development and application of chemical probes to globally map quinolone binding proteins. The revealed quinolone interactome contains both known as well as newly identified virulence factors and presents new targets for the treatment of bacterial infections.
Keyphrases
- pseudomonas aeruginosa
- biofilm formation
- cystic fibrosis
- acinetobacter baumannii
- endothelial cells
- poor prognosis
- high density
- high resolution
- escherichia coli
- candida albicans
- staphylococcus aureus
- induced pluripotent stem cells
- single molecule
- fluorescence imaging
- living cells
- antimicrobial resistance
- binding protein
- dna methylation
- nucleic acid
- fluorescent probe