Immunostaining for Epigenetic Modifications in Fagopyrum Calli.

Alicja Tomasiak Katarzyna Sala Agnieszka Braszewska

Published in: Methods in molecular biology (Clifton, N.J.) (2024)

Immunostaining is a well-established technique for identifying specific proteins in tissue samples with specific antibodies to identify a single target protein. It is commonly used in research and provides information about cellular localization and protein expression levels. This chapter describes a detailed protocol for immunostaining fixed Fagopyrum calli embedded in Steedman's wax using nine antibodies raised against histone H3 and H4 methylation and acetylation on several lysines and DNA methylation.

Keyphrases

dna methylation
genome wide
gene expression
randomized controlled trial
health information
binding protein
protein protein
copy number
social media