Amplification-free long-read sequencing reveals unforeseen CRISPR-Cas9 off-target activity.
Ida HöijerJosefin JohanssonSanna GudmundssonChen-Shan ChinIgnas BunikisSusana HäggqvistAnastasia EmmanouilidouMaria WilbeMarcel den HoedMarie-Louise BondesonLars FeukUlf GyllenstenAdam AmeurPublished in: Genome biology (2020)
Amplification-free long-read sequencing reveals Cas9 cleavage sites in vitro that would have been difficult to predict using computational tools, including in dark genomic regions inaccessible by short-read sequencing.